Trypsin Revisited
نویسندگان
چکیده
From the ‡European Molecular Biology Laboratory (EMBL) Hamburg c/o DESY, D-22607 Hamburg, Germany, the **Institute of Bioorganic Chemistry, Polish Academy of Sciences, 61–704 Poznan, Poland and Institute of Biochemistry and Molecular Biology, University Hospital, Hamburg-Eppendorf, c⁄o DESY, 22603 Hamburg, Germany, the ¶Laboratoire de Cristallographie et Modelisation des Matériaux Mineraux et Biologiques LCM3B CNRS UHP Faculté des Sciences, 54506 Vandoeuvre-les-Nancy Cedex France, and the Novozymes Protein Design, 2cs.01 DK-2880 Bagsvaerd, Denmark
منابع مشابه
Kunitz-type soybean trypsin inhibitor revisited: refined structure of its complex with porcine trypsin reveals an insight into the interaction between a homologous inhibitor from Erythrina caffra and tissue-type plasminogen activator.
The Kunitz-type trypsin inhibitor from soybean (STI) consists of 181 amino acid residues with two disulfide bridges. Its crystal structures have been determined in complex with porcine pancreatic trypsin in two crystal forms (an orthorhombic form at 1.75 A resolution and a tetragonal form at 1.9 A) and in the free state at 2.3 A resolution. They have been refined to crystallographic R-values of...
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Antimicrobial therapy remains to be the most important method of wound infection treatment. Systemically administered antimicrobials may not achieve therapeutic level in wound. On the other hand, in the absence of surgical debridement (due to any reason), most topically applied antimicrobials cannot penetrate the wound in therapeutic amount due to the presence of eschar. Burn eschar is a protei...
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The development of the marine aquaculture industry has led to the generation of significant amounts of fish wastes. Marine farm wastes exert adverse effects on the surrounding area of the cages. On the other hand, wastes of fish and other aquatic animals are regarded as major sources of valuable natural bioactive compounds, including enzymes, proteins, bioactive peptides, oil, amino acids, coll...
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Trypsin (E.C.3.4.21.4) is a serine protease commonly used in proteomics for digestion of proteins. In the present study, the effect of nano-TiO2 on the conformation and catalytic activity of trypsin were studied. The thermal denaturation of trypsin has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373 K) at pH 3.0 and 7.25, using temperature scanning...
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We give an overview of the running time analysis of the random divide-and-conquer algorithm FIND or QUICKSELECT. The results concern moments, distribution of FIND’s running time, the limiting distribution, a stochastic bound and the key: a stochastic fixed point equation.
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